The objective of this study was to quantify the necromass stock in a Mixed Ombrophilous Forest (MOF) fragment in the National Forest of Irati, State of Paraná, Brazil. Two sampling methods were tested: FA1, consisting of a fixed area (FA) approach with sample units measuring 2,500 m2 (50 m × 50 m); and FA2, consisting of fixed area sampling units measuring 500 m2 (10 m × 50 m) and line intercept sampling (LI) using 50 m lines. Data were collected on permanent sample plots installed in the area, consisting of 25 square blocks of 1 ha. Fallen dead wood pieces with a diameter = 10 cm were used in the analysis. The dead wood was classified into three degrees of decomposition, and masses were calculated as the corresponding density at each class. The tested sampling methods were evaluated using coefficient of variation and relative sampling error, and the nonparametric Kruskal-Wallis test was used to compare the results between the methods. Volume size of fallen dead wood did not statistically differ between the methods, but variation in necromass volume was lower in the FA1 method, whereas the FA2 method had a smaller sampling error. Overall sampling error ranged from 23.4-27.92%; lowering the sampling error to 15% would require a high sampling intensity (FA1: 42 area units a.u., FA2: 99 a.u., and LI: 236 a.u.). Total necromass weights amounted to 4.67 Mg.ha-1 (FA1); 5.16 Mg.ha-1 (FA2) and 4.58 Mg.ha-1 (IL), and carbon stock estimates were 2.00 Mg.C.ha-1 (FA1); 2.20 Mg.C.ha-1 (FA2) and 1.96 Mg.C.ha-1 (IL).

Biochar is a solid material formed during biomass thermochemical decomposition processes. This organic compound has particular properties that may cause effects on soils depending on its feedstock and processing conditions. Thus, the characteristics and purpose of use of this material must be recognized prior to its use. Two types of biochar, derived from different wood sources, were compared, one from caatinga biome species and another from cashew trees. Two species from caatinga biome were used, jurema-preta (Mimosa tenuiflora Willd. Poir.), and marmeleiro (Croton sonderianus Müll. Arg.). This study aimed to identify the best biochar material regionally available to increase water-holding capacity in the soil, based on laboratory tests and microstructural porosity evaluation. Biochar from Caatinga wood demonstrated an improved water-holding capacity if compared to cashew wood biochar. The particle diameters of 2 and 4 mm showed the highest levels, which were 2,268 g.g-1 for caatinga wood and 0.574 g.g-1 for cashew wood biochars, respectively. While the smaller quantities of macropores and a larger number of micropores (smaller radius) could explain the higher water-holding capacity for biochar from caatinga wood, the thick lignified cell walls of biochar from cashew wood support the idea of a hydrophobic effect contributing to water lower holding capacity.

Here we aimed to evaluate the influence of cladode morphology on the canopy formation of forage cactus plants. The study was carried out in Serra Talhada, the State of Pernambuco, using the IPA Sertânia (IPA), Miúda (MIU), and Orelha de Elefante Mexicana (OEM) clones, which were submitted to three irrigation depths (2.5, 5.0, or 7.5 mm) and three irrigation intervals (7, 14, or 28 days) from March 2012 to August 2013. Cladode and plant canopy biometric data were obtained during the experimental period. We found that the characteristics of the second and third order cladodes contributed most to the canopy formation of clones of the genus Nopalea (IPA and MIU), whereas, for the genus Opuntia (OEM), the first and third order cladodes contributed most. Overall, we found that the influence of the cladode variables on the canopy growth of forage cactus is more associated with the characteristics of the genus than to the peculiarities of the clones.

The most-used preparation process of protein isolates (PI) involves the isoelectric precipitation of the protein. Heating shortens the preparation time but this procedure may affect the purity, yield, molecular profile of the protein, and the activity of the trypsin inhibitor. This study aimed to investigate the effect of heating in the production of cowpea protein isolates. Crude whole beans (WB) were defatted with hexane, and the protein isolates obtained by isoelectric precipitation with (HPI), and without (NHPI) heating. The protein content of the WB and the PI was determined by the micro-Kjeldahl method, and the extraction yield estimated from the protein content at the end of extraction in relation to this content in the raw material. Possible losses of protein fractions were followed by SDS-PAGE, and the trypsin inhibitor activity determined by an enzymatic assay (BAPNA: benzoyl-DL-arginine-p-nitroanilide). Protein content in HPI was 83.3%, less than in the NHPI (92.2%). The HPI yield was lower (40.0%) as compared to the NHPI (42.3%). Electrophoresis indicated bands ranging from 13 to 262 kDa in WB; and the NHPI presented a protein fraction’s profile closer to that of the WB than to the HPI. The WB had the trypsin inhibitor activity, expressed as Trypsin Inhibitory Units (TIU), of 32.5±0.5 TIU /mg-protein; HPI showed 12.7±0.5 TIU /mg-protein (39% of that observed in WB) and the NHPI, 8.3±0.2 TIU /mg-protein (25.5%). Heating reduces the yield and purity of proteins in the isolates. However, the inhibitory activity of trypsin cowpea is most affected by the isolation procedure.

The high luminosity rates and high annual average temperatures of the Brazilian semiarid region, combined with the use of irrigation and adaptability of the sunflower crop to local climate, favor increased achene yield due to acceleration of morphophysiological processes. The objective of this work was to evaluate the morphophysiological and productive characteristics of sunflower varieties grown under drip irrigation in the Sub-Mid São Francisco River Valley. The experiment was conducted in the Experimental Field of the Brazilian Agricultural Research Corporation (Embrapa Semiarid), in Petrolina, State of Pernambuco, Brazil, in a randomized block design, with 21 sunflower varieties and four replications. The variables evaluated were flowering time, plant height, number of live leaves, stem and capitulum diameter, number of lodged and broken plants, stem curvature, relative chlorophyll index, photosynthesis, stomatal conductance, transpiration, average leaf temperature, 1000-achene weight and achene yield of the sunflower varieties. The treatments presented flowering time of 43 to 59 days after sowing, plant height of 1.0 to 1.4 m, capitulum diameter of 0.154 to 0.221 m, chlorophyll content of 30.8 to 33.98 Spad units and 1000-achene weight of 35.61 to 80.30 g. The sunflower varieties V7 and V8 stood out, with achene yields above 2,960 kg ha-1 and low number of lodged and broken plants, indicating a greater adaptability irrigation crops in the Sub-Mid São Francisco River Valley.

The aim of this study is to document the heretofore unrecorded natural occurrence of the larval parasitoid Digonogastra sp. on Diatraea flavipennella Box, 1931 (Lepidoptera: Crambidae) in sugarcane fields. The collections were made in the Usina Santa Clotilde area, in Rio Largo municipality, Alagoas State, Brazil. This is the first record of Digonogastra sp. parasitizing caterpillar of D. flavipennella in Brazil. The parasitism rate was found to be 14.06%. The occurrence of this parasitoid indicates potential for natural regulation of that pest in sugarcane crop.

Some forages release allelopathic substances into the environment, and may prevent consortium with arboreal species in pastures. The objective of this work was to evaluate photosynthetic characteristics of pequizeiro plants (hereafter pequi) influenced by concentrations of residues of the forage species Urocholoa decumbens, Melinis minutiflora and Paspalum notatum. The treatments consisted of pequi cultivation under aerial residues of the three forage species mixed to the substrate in four concentrations (1, 2, 3 and 4% mass/mass), plus an additional treatment (comparative control) with pequi cultivated on the substrate without waste. The following parameters were evaluated: photosynthetic rate, stomatal conductance, transpiration rate, relationship between internal and external CO2 concentration (Ci/Ca), maximum quantum yield, effective quantum yield of FS II, electron transport rate, nonchemical quenching and chlorophyll index, concerning the content of chlorophyll a, b and total in pequi plants at 50 and 100 days after transplanting (DAT), and the relative production of dry matter at 100 DAT. At 50 DAT, the following photosynthetic variables were affected in pequi plants: A, gs, chlorophyll a, chlorophyll b and total chlorophyll when cultivated in the presence of forage residues. At 100 DAT, the following photosynthetic variables were affected in pequi plants: A, Fv/Fm, ETR, NPQ, chlorophyll a, chlorophyll b and total chlorophyll, when cultivated in the presence of forage residues. Pequi plants had reduced relative dry matter yield when grown in the presence of U. decumbens. This variable was also affected when pequi was grown in increasing concentrations of residues of the species U. decumbens, M. minutiflora and P. notatum.

The objective of this work was to assess the effect of vegetation on the runoff coefficients of a Caatinga dry tropical forest before and after thinning. Thus, an experiment was conducted with evaluations in three hydrological years (2008, 2011 and 2013) in Iguatu, State of Ceará, Brazil. In 2008, the vegetation consisted of a 30-year regenerating Caatinga forest. The vegetation was subjected to thinning in 2009, 2011 and 2013, removing trees with less than 10-cm diameter at breast height. Hydrological responses were evaluated as a function of daily precipitation water depths, based on cumulative frequency distribution, by dividing precipitation events into three classes (CP) (CP≤30, 30<CP≤50 and CP>50 mm). Significant differences between runoff coefficients before and after vegetation thinning were assessed through the Student's t-test (p<0.01). Before thinning (2008), CP≤30 mm showed the highest runoff coefficient, differing statistically (p<0.01) from the other years. The results of precipitation events of great magnitude (CP>50 mm) indicate that the runoff is greatly dependent on rainfall characteristics and soil moisture conditions. The greater development of herbaceous vegetation due to thinning reduced the surface runoff.

Changes in the agricultural environment can be determined by providing microbiological indicators of the soil since the soil microorganisms are sensitive to variations in the environment. In this way, the impact of herbicides of long residual effect on the rhizospheric soil microorganisms of sugarcane cultivars was evaluated. The cultivars of sugarcane (SP 81-3250 and RB 867515) were treated with four herbicides (tebuthiuron, diuron, ametryn and mixture of diuron + and hexazinone) applied in pre-emergence. The herbicides were applied seven days after the planting of the gems. At 30, 60 and 90 days after the application, the soil rhizosphere was collected in each treatment to determine the CO2 evolution of the soil (C-CO2), microbial biomass carbon (MBC), metabolic quotient (qCO2), solubility potential of inorganic phosphorus P (PSFI) and percentage of roots colonized by mycorrhizal fungi Arbuscular. No changes were observed in the microbial activity of the sugarcane rhizosphere at 30 days after application of the herbicides (DAA). However, at 90 DAA, all herbicides negatively affected the activity of the rhizospheric microorganisms of sugarcane. The metabolic activity of rhizosphere in soil cultivated with RB 867515 was less affected by herbicides.

Enzyme activity in solid-state fermentation complex added to diets before and after extrusion process and its influence on Nile tilapia (Oreochromis niloticus) performance were evaluated. The following enzymes were analyzed: b-glucosidase, pectinase, xylanase, endoglucanase, amylase, protease, and phytase. The fish were fed five experimental diets: a control diet (without enzyme), two test diets supplemented before extrusion (400 and 800 ppm SSF), and two test diets after extrusion or “on top” (400 and 800 ppm SSF). The experiment had a completely randomized design with four replicates and seven fish per tank. Average initial weight was 10.37 ± 0.95 g, and fish were fed four times a day to satiation for 56 days. The following performance parameters were evaluated: final weight, weight gain, feed conversion, survival, specific growth rate, and body composition. The results were compared using ANOVA and Tukey’s test at p<0.05. All the enzymes showed partial or total losses of activity when subjected to the extrusion process, except phytase. No differences were observed for survival, water quality parameters, and body composition. On-top inclusion provided a better performance, which is observed through increases in final weight, weight gain, and feed conversion. As a result, 800 ppm of enzyme complex SSF should be added "on top", that is after extrusion, to improve tilapia performance and, consequently, contribute to the fish farming financial efficiency.