HMDO-Promoted Peptide and Protein Synthesis in Ionic Liquids

Duan, Jianli; Sun, Yao; Chen, Hao; Qiu, Guofu; Zhou, Haibing; Tang, Ting; Deng, Zixin; Hong, Xuechuan

HMDO-Promoted Peptide and Protein Synthesis in Ionic Liquids

2013

Hexamethyldisiloxane (HMDO) has been developed to efficiently promote the metal-free direct coupling of an amino function of one cysteine-free peptide or protein and a C-terminal thioester of the second peptide in ionic liquids. The amide-coupling reaction proceeds smoothly under mild conditions to afford the corresponding products in good to excellent yields (63–94%). Peptide couplings were also achieved using in-situ-generated thioesters by the thioesterification of oxo esters.

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