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Biochemical properties of new varieties of rape
2014
Рудник-Іващенко, О. І | Шовгун, О. О | Іваницька, А. П | Щербиніна, Н. П | Ляшенко, С. О | Чухлєб, С. Л | Бадяка, О. О
100 rape varieties and hybrids of domestic and foreign selection that have been grown in the different soil-climatic zones of the country were evaluated. The paper presents the results of study by their biochemical characteristics: content of protein, oil and its fatty acid composition. High-oil varieties of rape are identifi ed with high content of erucic acid in oil – for the technical purposes and those without or with its low content – for food industry.
显示更多 [+] 显示较少 [-]Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.)
2014
Король, Л. В | Гончарова, С. О | Піскова, О. В | Костенко, А. В | Коровко, І. І | Кожемякіна, Л. М
This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.
显示更多 [+] 显示较少 [-]Biochemical properties of new varieties of rape
2014
О. І. Рудник-Іващенко | О. О. Шовгун | А. П. Іваницька | Н. П. Щербиніна | С. О. Ляшенко | С. Л. Чухлєб | О. О. Бадяка
100 rape varieties and hybrids of domestic and foreign selection that have been grown in the different soil-climatic zones of the country were evaluated. The paper presents the results of study by their biochemical characteristics: content of protein, oil and its fatty acid composition. High-oil varieties of rape are identifi ed with high content of erucic acid in oil – for the technical purposes and those without or with its low content – for food industry.
显示更多 [+] 显示较少 [-]Peculiarities of the genetically modified organisms’ detection in seed batches of rape (Brassica napus L.)
2014
Л. В. Король | С. О. Гончарова | О. В. Піскова | А. В. Костенко | І. І. Коровко | Л. М. Кожемякіна
This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.
显示更多 [+] 显示较少 [-]