Purpose. To study the effect of AKM plant growth regulator on growth, development and yield formation of sunflower (Helianthus annuus L.) hybrids in hybridization plots under the conditions of the Southern Steppe zone of Ukraine. Methods. Laboratory tests, field study, statistical evaluation. Results. The results of studies devoted to determining the optimal AKM concentration for the treatment of seeds of the maternal and paternal lines, the effect of AKM on field germination, biometric parameters of sunflower plants, seed quality (F1) and yield are presented. Three hybrids of the Ukrainian selection, such as ‘Alpha’, ‘Logos’ and ‘Persei’ were studied during 2014–2016. Optimal concentration of AKM (0.0015 g/l) was defined. The vigor of seeds processed by AKM was higher than in check variety by 0.8–12.8 р.р. (♂); 0.4–10.7 p.p. (♀), laboratory germination – by 2.3–6.1 p.p. (♂); 3.5–6.2 p.p. (♀). In 2016, the sunflower plant height for all variants exceeded this parameter to be obtained for other years of the study. This could be explained by the fact that HTC in 2016 for the BBCH 00–39 period was 1.4 times higher than in 2015. In general, hybrids as the studied factor considerably influenced sunflower yield, and the share of the hybrid (factor A) influence is 33%. This should be considered when selecting hybrids for sunflower cultivation technologies in the Steppe zone of Ukraine. Conclusions. Hydrothermal conditions of the year had the maximum impact on the formation of seed quality and yield of sunflower plants of the hybrids under study, but when using AKM growth regulator for presowing seed treatment, this negative impact was reduced by an average of 23%.

Purpose. To identify and describe the basic criteria for studying and assessing decorative and economic-and-biological characters of the genus Heuchera L. varieties in the context of introduction. Methods. Introduction procedure, analytical approach, morphological analysis and biometric data evaluation. Results. Aided by the comparative morphological analysis of introduced varieties of the genus Heuchera, significant differences in their characters were revealed, including plant height, height and width of the basal rosette of leaves, generative shoot height. There was a significant variation of such parameters as the number of generative shoots within one plant, the number of flowers on a single generative shoot, flowering abundance. Based on investigation findings, 17 groups of varieties were identified for the dominant color of adaxial surface of the leaf blade and 7 groups – for inflorescence color. According to phenological observations, Heuchera varieties groups were determined and the dates of commencement and duration of plants flowering were registered in the context of introduction. The results of these investigations are the necessary basis for the study and evaluation of this culture assortment, they are important in breeding and landscaping. Conclusions. Based on the results of investigations of morphological features of introduced species of the genus Heuchera, traits and parameters for varieties grouping were defined as well as decorative and economic-and-biological traits that should be evaluated.

Purpose. To establish the reasons of submitting not highly decorative lilac hybrids to state variety testing so that unpromising varieties and look-alikes can be sometimes included in the world collection, and focus on solving this situation. Methods. Analytical approach. Results. It was established why and how unoriginal hybrids were submitted to state variety testing that for several reasons can allow them to obtain the status of variety. Conclusions. In order to prevent obtaining the status of variety by some low decorative lilac hybrids, it is necessary that both a breeder and testing stations employer should use known presentable collections of identified varieties for evaluation of real value of a new hybrid. In Ukraine, there is such a collection at N. N. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine where more than 130 varieties and 21 species of the genus Syringa occupies the area of 2.35 ha. It is desirable to compare not only with the check variety that the breeder selected for his hybrid among such, but, if it is possible, with all varieties in the collection close to it by decorative effect. The problem is that the author of a hybrid, sometimes ignorantly or for some other reason, selects a check variety for his hybrid that is not the most decorative one among such, against which a candidate for variety status will be more attractive during its evaluation. In such a case, the breeder should change a check variety that permits to estimate really the decorative effect of a submitted hybrid.

Purpose. To review publications relating to the key point of the genotyping technology that is competitive allele-specific polymerase chain reaction (which is called now Kompetitive Allele Specific PCR, KASPTM) and its use in various genetic-breeding researching (a study of maize). Results. The essence of KASP-genotyping, its advantages are highlighted. The requirements for matrix DNA are presented, since the success of the KASP-analysis depends on its qua­lity and quantity. Examples of global projects of plant breeding for increasing crop yields using the KASP genoty­ping technology are given. The results of KASP genotyping and their introduction into breeding and seed production, in particular, for determining genetic identity, genetic purity, origin check, marker-assisted selection, etc. are presented using maize as an example. It is demonstrated how geno­mic selection according to KASP genotyping technology can lead to rapid genetic enhancement of drought resistance in maize. Comparison of the effectiveness of creating lines with certain traits (for example, combination of high grain yield and drought resistance) using traditional breeding approaches (phenotype selection) and molecular genetic methods (selection by markers) was proved that it takes four seasons (two years in case of greenhouses) in order to unlock the potential of the plant genotype using traditional self-pollination, test-crossing and definitions), while using markers, the population was enriched with target alleles during one season. At the same time, there was no need for a stress factor. Conclusions. KASP genotyping technology is a high-precision and effective tool for modern genetics and breeding, which is successfully used to study genetic diversity, genetic relationship, population structure, gene­tic identity, genetic purity, origin check, quantitative locus mapping, allele mapping, marker-assisted selection, marker-assisted breeding. It is expedient and timely to introduce KASP genotyping technology in our country to solve a wide range of modern genetics, breeding, seed production tasks.

Purpose. To determine the effect of the basic organo-mineral fertilizer on the formation and stability of grain quality of winter wheat varieties in multiple crop rotation after black fallow as a predecessor. Methods. Field experiments were based on a multifactorial scheme using split-plot method with due regard to all requirements of the field experiment procedure, analysis of variance was used for statistical processing of the obtained results. Results. Investigation data was given concerning determination of grain quality indices in winter wheat varieties of diffe­rent ecotypes after black fallow as a predecessor depending on organo-mineral fertilizer application in the Left-Bank Forest-Steppe zone of Ukraine. In average for the period of investigation (2011–2015), the highest protein content in winter wheat grains was formed in no treatment variant [in such varieties as ‘Doridna’ (14.1%), ‘Dykanka’ (14.3%) and ‘Levada’ (14.2%)] and in case of organo-mineral fertilizer application [in the varieties ‘Hordovyta’ (14.0%), ‘Kalyta’ (14.0%), ‘Dykanka’ (14.7%) and ‘Levada’ (14.6%)]. The highest content of crude gluten in grains, without regard for the variant of the experiment, was found in the following varieties as ‘Dykanka’ (24.9–25.1%) and ‘Levada’ (23.7–25.4%). Conclusions. It was established that the content of protein and crude gluten in grains as well as the falling number of winter wheat was highly dependent on such factors as the variety and the year of cultivation as compared to the fertilizer background. The following varieties as ‘Hordovyta’, ‘Mulan’, Dykanka’ and ‘Levada’ were very sensitive to the application of organo-mineral fertilizer for the protein content, while ‘Hordovyta’ (2.4%), ‘Levada’ (1.7%), ‘Borvii’ (1.2%) and ‘Mulan’ (1.1%) – for the crude gluten content.

Purpose. To make comprehensive assessment of introduced oat samples of various eco-geographical origin under the conditions of the Forest-Steppe zone of Ukraine for the set of productivity and adaptability indices in order to define the most valuable ones and describe them. Methods. Field study, laboratory analysis, generalization. Results. The authors presented results of the study, evaluation and description of 35 new samples of oats from 5 countries for productivity and adaptability traits during 2011–2015 in Ustymivka Experimental Station for Plant Production to be run by the V. Ya. Yuriev Institute for Plant Production of NAAS. In field and laboratory conditions, the following indicators as yielding, productivity, thousand-kernel weight, early ripening, height of plants and length of panicle, lodging resistance were stu­died. Conclusions. A set of studies of new oat samples under various weather conditions allowed to identify material with increased parameters of economic and biological characters. During studies, it was found that ‘Laima’, ‘Stendskaia Darta’ (Latvia), ‘Borowiak’ (Poland), ‘Faks’ (Belarus) were the high-yielding varieties. Panicles in such oats samples as ‘Cwal’, ‘Borowiak’ (Poland), ‘Iakov’, ‘Mutika 1077’, ‘Mutika 990’, ‘Irtysh 22’, ‘Pokrovskyі 9’ (Russia), ‘Faks’ (Belarus), ‘Stendskaia Darta’, ‘Stendskaia Mara’ (Latvia) contained a large mass of grains (more than 2,5 g), panicle productivity in these samples was rather high both due to increased amount of grains in it and at the expense of thousand-kernel weight. Analysis of the study results showed that introduced oat samples of various eco-geographical origin were adapted to the Southern Forest-Steppe zone and can be recommended as an initial material in breeding for increasing productive and adaptive capacity.

Purpose. To investigate the variability of plant biometrics of cutting lettuce (Lactuca sativa L.) varieties depending on the concentration of microfertilizer Avatar-1. Methods. Field study, biometric technique, comparative approach, statistical evaluation, generalization. Results. Dependence of the plant height, the diameter of the leaf rosette, the number of leaves per plant, the leaf area of plants on the concentration of microfertilizer Avatar-1 was defined. Investigations of cutting lettuce ‘Afitsyon’ and ‘Concord’ varieties by Dutch breeding were conducted in 2016–2017 in the collection sites of the department of vegetable growing in the scientific-experimental field  “Fruit and vegetable garden” of the National University of Life and Environmental Sciences of Ukraine. In order to determine dependence of plant biometrics of cutting lettuce varieties on the concentration of complex microfertilizer Avatar-1, the following scheme was used for the both varieties: variant 1 – water (control); variant 2 – 0,10% solution; variant 3 – 0,25% solution; variant 4 – 0,50% solution. Plants were treated with microfertilizer three times during the vegetative period. Alterations of plant biometrics of cutting lettuce ‘Afitsyon’ and ‘Concord’ varieties  depending on the concentration of complex microfertilizer Avatar-1 was studied. Conclusions. It was found that in case of three-time plant treatment with complex microfertilizer Avatar-1 at the time of harvesting, the best plant biometrics was registered in variant 3 (concentration 0,25%) for the both ‘Afitsyon’ and ‘Concord’ varie­ties of cutting lettuce. The height of plants of the cutting lettuce in ‘Afitsyon’ variety exceeded this figure in ‘Concord variety by 1,1–1,4 cm. The concentration of microfertilizer had no significant effect on the diameter of the leaf rosette of ‘Concord’ variety (25,1–25,9 см). The diame­ter of the leaf rosette of ‘Afitsyon’ variety was 26,0–28,7 cm. In cutting lettuce, the largest leaf area per plant was registered in ‘Afi­tsyon’ variety (3516.5 cm2/plant ) in case of plants  treatment with 0,25% solution of complex microfertilizer Avatar-1 that exceeded this figure in ‘Concord’ varie­ty by 660 cm2/plant. The best plant biometrics of cutting lettuce of the studied varieties and optimal concentration of complex microfertilizer Avatar-1 (0.25% solution) was defined that is important for improving agricultural cultivation techniques.

Purpose. To substantiate and develop breeding technologies of soybean cultivation under various weather conditions of the Western Forest-Steppe zone. Methods. Laboratory test, statistical and mathematical analysis. Results. Analysis of the data of yielding increase due to the use of seed inoculation, green manure and spraying of crops with microbial prepa­rations showed that their effect was much dependent on the weather conditions during the year. Based on the analysis of productivity of such early ripening varieties of soybean as ‘Lehenda’, ‘Anzhelika’ and ‘Ksenia’ during 2011–2015, it was found that the influence of agrometeorological conditions during the vegetation period accounted for 47.8%. The results of the analysis of five-year data of productivity of the late soybean variety ‘Heorhina’ indicated that the share of influence of agrometeorological conditions during the vege­tation period on the studied variety was 48.8%. The use of microbial strains of nodule bacteria Bradyrhizobium japonicum 634b, 614A and M-8 against two backgrounds (in case of green manure application and without it) was compared, and microbial culture Hetomik application during the vege­tation period. Conclusion. All biological preparations and green manure considerably increased yield of soybean seeds in moderately humidified and elevated temperature conditions. For soybean growing, the application of green manure, seed inoculation with strains of M-8, 614A and Hetomik spraying of crops was effective. The use of these methods for gro­wing ‘Lehenda’ variety was the most effective. Such varieties as ‘Lehenda’ and ‘Anzhelika’ showed more stable results as compared to others in case of considerable variations of agrometeorological conditions.

Purpose. To investigate rice blast resistance genes polymorphism by using bioinformatic methods. Methods. Global and local nucleotide alignment, phylogenetic analysis, HyPhy test. Results. For Pib gene, numerous single nucleotide substitutions and deletions of 1–3 bp were established. The phylo­geny of this gene has been studied and homologues have been found both in various rice species and in other cereals. These sequences can encode proteins that «recognize» the phytopathogens effectors, and can also be associated with resistance to phytopathogens. The Pi4 gene is characterized by single nucleotide substitutions, insertions and deletions; the number of non-synonymous substitutions exceeds the number of sy­nonymous ones. The Pi54 gene variability is significantly lower than that of the Pi4 and Pib genes. The predominant types of polymorphism were single nucleotide substitutions and small-sized indels. It was found that non-synonymous substitutions in Pi54, Pi4 and Pib genes were in close proximity, sometimes forming clusters, while some coding regions were either superconservative or contained predominantly synonymous substitutions. On philodendrograms, cultivated rice samples were clustered with samples of ancestral wild-growing species. Conclusions. Evolution of the rice blast resistance genes Pi4, Pib and Pi54 is characterized by diversification selection. Considering that tense coevolution and significant rate of adaptation and creation of new pathogen races are typical for a plant and a parasite, these genes are subjected to intensive selection aimed at increasing diversity for obtaining the resistance to new races of the pathogen.

Purpose. To evaluate the stability and plasticity of new varieties of grain sorghum in ecological conditions of the Fores-Steppe and Steppe zone of Ukraine. Methods. Field study, statistical evaluation. Results. It was found that in Ukraine the average yield of grain sorghum in 2015 was 3.72 t/ha and in 2016 – 3.36 t/ha. In field experiments during 2009–2016, ‘Praim’ and ‘Sprynt 2’ hybrids appeared to be significantly better as compared to averaged yields of 3.9 t/ha. Evaluation of yield plasticity in environmental conditions of the Forest-Steppe and Steppe zones showed that the hybrid ‘Praim’ did not differ from the group standard and its index is no more than one. At the same time, the ‘Sprynt 2’ is highly-plastic hybrid and ‘Dniprovskyi 39’ belongs to relatively low-plastic varieties. Conclusions. It was established that, as an average of an experiment, hyb­rids of grain sorghum ‘Praim’ and ‘Sprynt 2’ had a yield of 4,2–4,3 t/ha. According to the analysis of ecological plasticity, ‘Sprynt 2’ can be included with the intensive type and ‘Dniprovskyi 39’ – with relatively low-plastic varieties. It was found that proper selection of varieties and far­ming practices of cultivation is one of the main factors that favors high yields and respectively determine 37% and 34% of the level of grain sorghum productivity.