Plastics have been widely reported to be present in the environment yet there are still many questions regarding the extent of this and the impacts these may have on both the environment and human health. The purpose of this investigation is to determine levels of micro and mesoplastic (MP), in the 1–5000 μm range, in commercially important species of finfish and shellfish. Additionally, to determine and compare the relative MP levels in edible versus non-edible tissues, and consider the wider implications in terms of human health concerns with a preliminary risk identification approach. For several fish species, samples taken from typically non-edible (gills, digestive system) and edible (muscle) flesh, and were analysed separately. Scallops, where all tissues are edible, were analysed whole. Significant differences were observed in the number of particles isolated from the finfish gills and digestive tissues relative to the control samples, but not in the edible flesh. For scallops, the abundance of particles in the Scottish samples did not vary significantly from the control, while the Patagonian scallops displayed significantly higher numbers of MPs. Characterisation of MPs by FTIR microscopy found that 16–60% (depending on species) were polyethylene terephthalate (PET) and polyethylene (PE) in origin. The risk identification results validate MPs as an emerging risk in the food chain and establish seafood as a vector for the exposure and uptake of MPs through the ingestion route for humans. Levels of MPs in seafood, and a direct link to the human food chain, suggests that their quantification be included as one food safety measure.

Lipids are the main energy support during gametogenesis. Digestive gland is the key organ of aquatic animal metabolism for storing nutrition and supplying energy. It participates in a variety of life activities (such as growth, digestion, immunity, and reproduction). Nutrients stored in digestive glands, especially lipids, provide energy for reproductive behaviors such as gametogenesis and ovulation. A large number of studies have confirmed the accumulation of lipids from digestive gland to gonad during gametogenesis. At present, the research on the interference mechanism of persistent organic pollutants (POPs) on lipid metabolism of aquatic animals and the adaptive response of aquatic animals to POPs stress focus on biochemical levels or a few genes. The potential molecular mechanism of lipid metabolism interference needs to be further studied. In addition, as an important stage of aquatic animals, the reproductive period is a vigorous period of lipid metabolism. However, at present, there is no report on the molecular mechanism of POPs interfering with the lipid metabolism of the digestive gland in the reproductive process of aquatic animals. In this study, female scallop C. farreri was cultured in natural seawater and exposed to 4 μg/L B[a]P in seawater. Transcriptome analysis of digestive glands at multiple stages (proliferative stage, growth stage, mature stage and spawn stage) was performed, and iPath pathway analysis was used to analyze lipid metabolism pathways and differential genes. The interference mechanism of lipid metabolism in bivalves during reproductive period was revealed. This study will provide valuable genomic information on the role of digestive glands in lipid metabolism and reproduction of C. farreri, and will contribute to further functional genomics of bivalves and other closely related species.

The effects of increasing concentrations of suspended mineral coal dust on the energetic physiology of the Caribbean scallop Argopecten nucleus were studied, at a concentration range that is environmentally relevant and representative of areas proximate to coal loading and shipping ports. Adult hatchery-produced animals were exposed to different concentrations of coal dust, i.e. 0, 2, 9 and 40 mg L⁻¹. At increasing concentrations of coal dust, the rates of filtration and pseudofeces production increased, while the rates of ingestion and absorption remained constant. The rates of oxygen consumption and ammonium excretion decreased, as well as the absorption efficiency and the scope for growth. Suspended coal dust particles, at concentrations higher than or equal to 2 mg L⁻¹, were ingested preferentially over microalgae by A. nucleus, causing reductions in its absorption capability, metabolism and in the amount of energy for growth and reproduction, thus generating physiological stress.

Lipophilic marine toxins in shellfish pose significant threats to the health of seafood consumers. To assess the contamination status of shellfish by lipophilic marine toxins in the Bohai Sea, nine species of shellfish periodically collected from five representative aquaculture zones throughout a year were analyzed with a method of liquid chromatography-tandem mass spectrometry (LC–MS/MS). Lipophilic marine toxins, including okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), yessotoxin (YTX), homo-yessotoxin (homo-YTX), azaspiracids (AZA2 and AZA3), gymnodimine (GYM), and 13-desmethyl spirolide C (13-DesMe-C), were detected in more than 95 percent of the shellfish samples. Toxins PTX2, YTX, 13-DesMe-C and GYM were predominant components detected in shellfish samples. Scallops, clams and mussels accumulated much higher level of lipophilic marine toxins compared to oysters. Toxin content in shellfish samples collected from different sampling locations showed site-specific seasonal variation patterns. High level of toxins was found during the stages from December to February and June to July in Hangu, while from March to April and August to September in Laishan. Some toxic algae, including Dinophysis acuminata, D. fortii, Prorocentrum lima, Gonyaulax spinifera and Lingulodinium polyedrum, were identified as potential origins of lipophilic marine toxins in the Bohai Sea. The results will offer a sound basis for monitoring marine toxins and protecting the health of seafood consumers.

The exposure risk of metal-based nanoparticles (NPs) to marine organisms and related food safety have attracted increasing attention, but the actual concentrations of these NPs in seawater and marine organisms are unknown. In this work, single particle inductively coupled plasma-mass spectrometry (spICP-MS) was used to quantify the concentrations and size distributions of NPs in different marine mollusks (oysters, mussels, scallops, clams, and ark shells) from an offshore aquaculture farm. Results showed that Ti, Cu, Zn, and Ag bearing NPs were detected in all the five mollusks with the mean sizes at 65.4–70.9, 72.2–89.6, 97.8–108.3, and 42.9–51.0 nm, respectively. The particle concentrations of Ti, Cu, Zn, and Ag bearing NPs in all mollusks (0.88–3.26 × 10⁷ particles/g fresh weight) were much higher than that in the seawater (0.46–0.79 × 10⁷ particles/mL), suggesting bio-accumulation of NPs. For all the five mollusks, Ag bearing NPs had the highest number-based bioconcentration factors (NBCFs) in all the tested NPs due to the smallest mean size of Ag bearing NPs in seawater (30.5 nm). In addition, the clams exhibited the lowest NBCFs of the four NPs than other mollusks. All four NPs were mainly accumulated in the gill and digestive gland, and could transfer to adductor muscle of all mollusks. Although all the four metals (Ti, Cu, Zn, Ag) in mollusks were safe for human consumption by the estimated daily intake (EDI) analysis, the risk of NPs remaining in the mollusks should be further considered when evaluating the toxicity of metals for human health. The findings could improve our understanding on the distribution and health risk of NPs in marine mollusks under offshore aquaculture.

Differences in the accumulation of mercury (Hg) in five species of marine bivalves, including scallops Chlamys nobilis, clams Ruditapes philippinarum, oysters Saccostrea cucullata, green mussels Perna viridis, and black mussels Septifer virgatus, were investigated. The bivalves displayed different patterns of Hg accumulation in terms of the body concentrations of methylmercury (MeHg) and total Hg (THg), as well as the ratio of MeHg to THg. Parameters of the biodynamics of the accumulation of Hg(II) and MeHg could reflect the species-dependent Hg concentrations in the bivalves. With the exception of black mussels, we found a significant relationship between the efflux rates of Hg(II) and the THg concentrations in the bivalves. The interspecific variations in the MeHg to THg ratio were largely controlled by the relative difference between the elimination rates of Hg(II) and MeHg. Stable isotope (δ¹³C) analysis indicated that the five bivalve species had contrasting feeding niches, which may also affect the Hg accumulation.

Uptake of waterborne Cd, Co, Mn and Zn was determined in laboratory experiments using radiotracer techniques (109Cd, 57Co, 54Mn and 65Zn). Labelled Zn was mainly accumulated in the digestive gland (65%) and Co in kidneys (81%); Cd and Mn were similarly distributed in digestive gland and gills. In a complementary field study, Ag, As, Cd, Co, Cr, Cu, Fe, Mn, Ni, and Zn were analysed in scallops collected at two stations showing different contamination levels. Digestive gland and kidneys displayed the highest concentrations. Ag, As, Cd, and Fe differed in soft tissues from the two stations, suggesting that Comptopallium radula could be a valuable local biomonitor species for these elements. Low Mn and Zn concentrations found in kidneys suggest that their content in calcium-phosphate concretions differs from the other pectinids. Preliminary risk considerations suggest that As would be the only element potentially leading to exposure of concern for seafood consumers. This study investigates metal accumulation behaviour in the tropical scallop Comptopallium radula and preliminary risk assessment for consumers.

The characterization of organic and inorganic environmental pollution in coastal ecosystems, such as port areas, is complex and difficult to carry out due to the effect of environmental variables, as well as anthropic activities. For this study, the objective was to define a statistical method, taking into account the confounding factors influence, to define reference values for biomarkers in the black scallop (Mimachlamys varia). Thus, for five biomarkers (SOD (Superoxyde Dismutase), GST (Glutathion-S Transferase), MDA (Malondialdehyde), AChE (Acetylcholinesterase) and LAC (Laccase)), reference data could be described for individuals placed on sites more or less strongly impacted by specific environmental contaminations in port areas for more than two years, which had never been done before. All these results enabled us to calibrate and validate our approach in terms of active biomonitoring for the evaluation of a good ecological status of the environment of a port located on the French Atlantic coast.

In recent years, dense Alexandrium ostenfeldii blooms have been reported in different coastal areas. In this study, we report for the first time the occurrence of A. ostenfeldii blooms associated with the detection of paralytic shellfish toxins (PSTs) in the Peruvian scallop (Argopecten purpuratus) from Paracas Bay. Alexandrium ostenfeldii blooms occurred at the end of summer and early fall, after the increase of riverine input and under stratified conditions following a decrease in wind velocity. The highest abundances occurred during warm sea surface temperatures (18–27 °C). High PST concentrations that exceed the maximum permissible level (800 μg STX eq. kg⁻¹) occurred even under low A. ostenfeldii abundances (20 × 10³ cells l⁻¹). Our results contribute to a better understanding of the dynamics of A. ostenfeldii in coastal systems influenced by riverine inputs and upwelling and can be used to improve monitoring programs and allow the implementation of mitigation measures along the Peruvian coast.

Marine seismic surveys are an important tool to map geology beneath the seafloor and manage petroleum resources, but they are also a source of underwater noise pollution. A mass mortality of scallops in the Bass Strait, Australia occurred a few months after a marine seismic survey in 2010, and fishing groups were concerned about the potential relationship between the two events. The current study used three field-based methods to investigate the potential impact of marine seismic surveys on scallops in the region: 1) dredging and 2) deployment of Autonomous Underwater Vehicles (AUVs) were undertaken to examine the potential response of two species of scallops (Pecten fumatus, Mimachlamys asperrima) before, two months after, and ten months after a 2015 marine seismic survey; and 3) MODIS satellite data revealed patterns of sea surface temperatures from 2006–2016. Results from the dredging and AUV components show no evidence of scallop mortality attributable to the seismic survey, although sub-lethal effects cannot be excluded. The remote sensing revealed a pronounced thermal spike in the eastern Bass Strait between February and May 2010, overlapping the scallop beds that suffered extensive mortality and coinciding almost exactly with dates of operation for the 2010 seismic survey. The acquisition of in situ data coupled with consideration of commercial seismic arrays meant that results were ecologically realistic, while the paired field-based components (dredging, AUV imagery) provided a failsafe against challenges associated with working wholly in the field. This study expands our knowledge of the potential environmental impacts of marine seismic survey and will inform future applications for marine seismic surveys, as well as the assessment of such applications by regulatory authorities.