Application of a solid-phase fluorescence immunoassay to determine streptomycin residues in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major)
2007
Chung, H.S. (Hapcheon Country Office, Hapcheon, Republic of Korea) | Kim, S. (Gyeongsang National University, Jinju, Republic of Korea) | Chun, M.S. (Seoul National University, Seoul, Republic of Korea) | Jung, W.C. (Gyeongsang National University, Jinju, Republic of Korea) | Kim, D.H. (Gyeongsang National University, Jinju, Republic of Korea) | Shon, H.Y. (Yangsan City Hall, Yangsan, Republic of Korea) | Lim, J.J. (Gyeongsang National University, Jinju, Republic of Korea) | Lee, H.J. (Gyeongsang National University, Jinju, Republic of Korea), E-mail: hujang@gnu.ac.kr
Parallux (IDEXX Laboratories, USA), a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of antibiotics in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Fishes were dipped in streptomycin 20 g/ton water, the recommended therapeutic dose, for 3 consecutive days. Muscle was sampled after 1st, 2nd, 3rd, 4th and 5th day drug treatment. The concentration of streptomycin in muscle, determined by SPFIA, was compared with that of internal standard (200 ppb as streptomycin).
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