Potato tuber starch characteristic is influenced by the ratio of amylose to amylopectin, which is affected by the granule-bound starch synthase (GBSS) gene. GBSS gene expression was decreased in the leaves of the Desiree potato variety utilizing genome editing using CRISPR-Cas9. Constructs encoding Cas9 gene and sgRNAs targeting GBSS gene were inserted into plant leaves using Agrobacterium-mediated transformation delivery. The results obtained lines with mutations in GBSS genes accounting for 21% of regenerated shoots. The identification of mutations within one base pair of the used guide sequence provided further evidence of the considerable similarity between the target region around the protospacer adjacent motif (PAM) position and the used guide sequence. Transforming DNA into potato leaves produced mutants that lacked the Cas9 gene. Using microscopic inspection of iodine-stained starch granules, the increase of amylopectin in the starch granules of editing potato tubers was evaluated.

Heat exchanger optimization is a very efficient thermal design inquiry. The goal of the research is to determine an optimal channel flow velocity as well as an optimal total plate number based on overall information on the effectiveness of employing chilled water to cool soybean milk. Heat transfer coefficient, Prandtl number, Reynolds number and pressure drop measurements were applied for milk and water. The rheological properties of soybean milk have been investigated at various temperatures (5, 10, 30, 50, 70 and 90 °C). The operational circumstances of PHE in soybean procedures were investigated at different numbers of plates, flow rates and flow velocities. Based on the present study, total substances showed non-Newtonian pseudoplastic conduct in any respect at any temperature and the power law model has a decent correlation. The cost estimation of the plate heat exchanger was observed at the lowest flow velocity of (2.25) m/s and number of plates is 17.

This study aimed to use the organic polymer, chitosan, as a flocculant for harvesting microalgae Chlorella. sp. Various polymer concentrations were tested. Within three min, 50 mg/L of chitosan showed the highest efficiency (98.9±0.5%), at pH 6.3, while the lowest efficiency (86.7±0.5%) was observed at pH 7.5. Harvesting efficiency in this study was chitosan concentration dependent. The present study showed that chitosan can be a promising and sustainable solution as an organic source and environmentally friendly flocculant. Additionally, it has excellent harvest productivity and requires minimum effort for quick outcomes.

Dairy cattle in Sudan are traditionally fed on separate concentrate and roughage diets. Because of the high cost of forages, feeding strategies should be attempted to reduce feeding costs. A traditional dairy ration (As-fed), composed of 49% sorghum grains (SG), 29.4% groundnut seed cake (GSC), 19.6% wheat bran (WB), 1% limestone (LS) and 1% salt (NaCl), was used as a control. The test ration was a total mixed ration (TMR) composed of 40% SG, 23% groundnut seed hulls (GSH), 20% GSC, 15% WB, 1% LS and 1 % NaCl. The control traditional ration was fed at 10 Kg/cow/day together with 7 Kg sorghum chaff. Two levels of TMR were used with alfalfa hay (AH); (17 Kg TMR + 1 Kg   AH/cow/day and 15 Kg TMR + 2 Kg AH. Three cross-bred dairy cows (European x Local) were used with the feeding regimes in a 3x3 Latin square design.  Cows consumed similar amounts of DM, CP and ME and produced similar quantities of milk (P > 0.05).  TMR had no effects (P > 0.05) on the composition of milk. The cost for TMR was decreased by 19%.TMR was thus effective in reducing feeding costs without affecting milk production and composition.

The honeybee, Apis mellifera, is a widespread pollinator insect. Colony collapse disorder (CCD) has recently caused a significant decline in honeybee numbers worldwide. The beekeeping industry, agriculture, and world biodiversity are threatened by the honeybee population decline. We examined the mRNA levels of heat shock protein gene 60 (hsp60), acetylcholinesterase 1, and acetylcholinesterase 2, as biomolecular markers of the insect response to three separate forms of environmental stress, including high temperature, low temperature, and insecticide. The obtained results indicated increased levels of hsp60 and acetylcholinesterase 2 after the exposure of worker bees to temperatures of 17, 32, and 40°C for one hour, as well as exposure to a low dose of pesticide for 24 h ingestion of imidacloprid solution (2 ppm); meanwhile, acetylcholinesterase 1 showed remarkable expression decrease under heat and cold stresses but increased under pesticide treatment.  The responses of honeybees to hsp60, acetylcholinesterase 1, and acetylcholinesterase 2 are diverse and very specific to various stimuli. According to this research, the distinct expression profiles of hsp60 and acetylcholinesterase 2 can serve as useful instruments for tracking honeybees' vulnerability to varied environmental effects. Due to its insufficient responsiveness, acetylcholinesterase 1 expression is not permitted for use as a biomarker.

The present study evaluated the ability of different extracts of cinnamon and black cumin seeds to inhibit the growth of the phytopathogenic fungus Rhizoctonia solani and its extracellular cell wall degrading enzymes. Concentrations of 300 and 450 ppm of methylene chloride and hexane extracts of cinnamon completely inhibited the growth of R. solani after 72 hours of incubation at 25±1°C in vitro. Methylene chloride or hexane extracts of black cumin seeds at 4000 ppm after 72 hours inhibited the growth of R. solani by 37 and 39% respectively. Moreover, black cumin seed hexane extract at 2000 ppm inhibited 55% and 38% of the activity of pectin lyase (PL) and polygalacturonase (PG) respectively. In addition, the methanolic extract of black cumin seeds at 2000 ppm exhibited a significant reduction of exo-protease activity (74.8%). GC-MS analysis results showed that linoleic acid is the main component of the fixed oil fraction of black cumin seed hexane extract while (E) cinnamaldehyde is the main component in both hexane and methylene chloride extracts of cinnamon. HPLC-MS analysis of black cumin seeds methanolic extract showed that amentoflavone was the main component. Docking was used to identify the major component interaction with pectin lyase A and exo-protease.

A study was carried out in a mango orchard on reclaimed land for two seasons in 2019 and 2020. The experiment was implemented on fifteen years old mango trees “Fagri Kalan” budded on seedling mango rootstock and planted at 4 × 4 m. This experiment involved two soil growth stimulants (K humate, magnetite) and foliar application with cobalt. The experiment involved two factors, the first one was soil application with K humate by two levels (50 and 100 g/tree/year) and magnetite by two levels (250 and 500 g/tree /year) plus control, whereas the second factor was a foliar application with Co as cobalt sulfate by three levels (0, 15, and 30 ppm Co). The experiment was carried out in a factorial experiment in split plot design with three replicates whereas each replicate was represented by two trees. Result indicated that soil application with K humate at 100 g/tree followed by magnetite at 250 g/tree or foliar application with Co at 15 ppm alone or the combination between (K humate at 100 g/tree and cobalt at 15 ppm) gave the highest values of yield/tree, fruit weight, TSS, reducing sugars, leaf N, P, K, and Fe content of “Fagri Kalan” mango trees grown under salinity conditions.

This study examined the macro and trace element contents as well as the cytotoxicity of Laurencia obtusa, Ulva lactuca, and Padina pavonica algae collected from the Mediterranean coast of Egypt (Alexandria coast). The results indicated that the seaweed samples contained high concentrations of Ca, Cl, Mg, K, Na, P and Fe. The Cu and Zn contents of the algae were in the ranges of 0.39-0.95 mg/100 g dw and 0.11-0.49 mg/100 g dw respectively. Fresh red algae contained the highest level of Zn and Cu, whereas the iodine level was 1.52, 1.27 and 1.01 mg/100g in fresh each of Padina pavonica, Laurencia obtuse and Ulva lactuca respectively. Fresh red, fresh green, dry green, dry red, in addition to fresh and dried brown algae had maximum lead (Pb) levels of 0.16, 0.14, 0.13, 0.12, 0.04 and 0.02 g/100 g respectively. Fresh red algae contained the highest Cd and Mn levels, whereas dried brown algae exhibited the lowest Cd and Mn concentrations. Different extracts of fresh and dried brown algae exhibited significant larvicidal activity against brine shrimp. LC50 values indicated that dry samples were more cytotoxic than fresh samples.

The experiments were conducted during seasons (summer, au-tumn, winter, and spring) 2020–2021 to study the activities of invertase, adenosine triphosphatase (ATPase), and alkaline phosphatase (AlkP) as well as the determination of total proteins. These activities were determined in the bodies of adult honeybee worker, Apis mellifera, (house and forager bees) after feeding on three different diet groups (A) pollen cake, (B) supplementary diet and (C) control group during the four seasons. Group (A) received 50 g pollen cake + 250 ml honey syrup (2 honey: 1 water)/colony/three-day intervals, group (B) received 50 g Brewer’s yeast chickpea cake fortified with 4.2% pollen + 250 ml sugar syrup (2 sugar: 1 water) while group (C) received 250 ml sugar syrup (1 sugar: 1 water) only. Results indicated that the highest increase in total proteins was found in group B during the autumn season with house bees. Invertase activity was high in group A during the summer season with forager bees. The highest ATPase activity was observed in group B during the spring season with house bees, whereas the highest AlkP activities were found in group A during the autumn season with house bees.

This study was conducted to investigate the effects of five essential oils (garlic, Allium sativum; camphor, Cinnamomum camphor; menthol, Mentha sp.; cinnamon, Cinnamomum erum; and clove, Dianthus sp.) on wax moth larvae and adult honeybee workers under laboratory conditions. Cinnamon, garlic, menthol, clove, and camphor oils were found to be highly effective against wax moth larvae, with 100 percent mortality achieved after 48 and 96 hours for cinnamon and garlic oils respectively, and after 120 hours for clove and mint oils. Camphor oil provided 96.60% accumulative mortality after 120 hrs. Moreover, all the essential oils were highly safe for adult honeybee workers under laboratory conditions. Accumulative corrected mortality rates after 72 hr were 15.96, 9.02, 13.18, 29.16, and 8.88% for treatments with cinnamon, clove, camphor, mint, and garlic oils respectively.