Enzymatic hydrolysis as a process influent of changing immunoreactive properties of sodium caseinate
2003
Wroblewska, B. | Karamac, M. (Polska Akademia Nauk, Olsztyn (Poland). Inst. Rozrodu Zwierzat i Badan Zywnosci)
The aim of the work was to determine the effect of enzymatic hydrolysis of sodium caseinate with Subtilisina carlsberg - Alcalase 2,4 L FG (Novo Nordisk) and papain - EC 3.4.22.2 (Sigma) on immunoactivity. The complex of analytical immunometric methods were applied with the use of polyclonal antibodies. The most beneficial effect was achieved during "two-step" hydrolysis (alcalase-papain) of sodium caseinate but it was impossible to achieve total reduction of immunoreactivity
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